Journal: Journal of Clinical Laboratory Analysis

Article Title: The up‐regulated hsa‐circRNA9102‐5 may be a risk factor for essential hypertension

doi: 10.1002/jcla.23339

Figure Lengend Snippet: CircRNA‐miRNA‐mRNA network diagram. The red circle represents the target circRNA hsa‐circRNA9102‐5, the blue circle represents the miRNA, and the yellow circle represents the target miRNA hsa‐miR‐150‐5p. The lines represent the connection and binding of each other

Article Snippet: Quantitative PCR was performed using the GoTaqqPCR Master Mix (Promega) and the miRcute Plus miRNA SYBR Green qPCR Detection Kit (TIANGEN) on a Roche LightCycler 480 Real Time PCR instrument (Roche), by following the manufacturers' instructions.

Techniques: Binding Assay

Journal: Cancer Science

Article Title: The Positive Feedback of lncRNA ANRIL / miRNA ‐339‐5p/ ZBTB7A Suppresses Metastasis of Nasopharyngeal Carcinoma Cells via SREBP1 ‐ FASN

doi: 10.1111/cas.70225

Figure Lengend Snippet: ANRIL/miR‐339‐5p/ZBTB7A axis regulates SREBF1‐FASN. (A) MiR‐339‐5p binding sites on ANRIL and ZBTB7A were predicted. (B) Luciferase activities of HK1 and 5‐8F cells co‐transfected with ANRIL WT/MUT or ZBTB7A WT/MUT and miR‐339‐5p mimic or mimic NC were respectively analyzed. (C) A potential interaction was predicted between ZBTB7A and the promoter region of ANRIL and (D) luciferase activities of HK1 and 5‐8F cells co‐transfected with ANRIL WT/MUT and ZBTB7A or NC‐ZBTB7A. (E) ChIP‐qPCR was used to analyze whether ZBTB7A is directly bound to the ANRIL promoter. (F) Potential interactions between ZBTB7A and the promoter region of SREBP1 or FASN were predicted. (G) Luciferase activities of HK1 and 5‐8F cells co‐transfected with SREBF1 WT/MUT or FASN WT/MUT and ZBTB7A or NC‐ZBTB7A. (H) ChIP‐qPCR was used to analyze whether ZBTB7A is directly bound to the SREBF1 or FASN promoter. * p < 0.05, ** p < 0.01, *** p < 0.001; ns , not significant.

Article Snippet: QPCR reactions were performed using FastStart Universal SYBR Green Master Mix (Roche, Switzerland) or a miRcute Plus miRNA qPCR Detection Kit (Tiangen) in a 7500 real‐time PCR system (Applied Biosystems, USA).

Techniques: Binding Assay, Luciferase, Transfection, ChIP-qPCR

Journal: Frontiers in Endocrinology

Article Title: Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait

doi: 10.3389/fendo.2023.1165409

Figure Lengend Snippet: Summary information of lncRNA, mRNA, and miRNA identification. (A) According to the identification results, we mainly divided lncRNA and mRNA into Known and Novel two types, and the pie chart was made according to the proportion. (B) The statistical results of unique clean reads from the uterine tissues in the two experimental groups. We distinguished small RNAs into 10 types as shown in the figure. (C) Chromosome distribution of identified mRNA and lncRNA from the uterine tissues.

Article Snippet: The expression of the selected mRNAs and miRNAs was quantitatively analyzed using a SYBR Green qPCR Mix Kit (TaKaRa) and miRcute Plus miRNA qPCR Kit (TIANGEN) according to the manufacturer’s instructions.

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait

doi: 10.3389/fendo.2023.1165409

Figure Lengend Snippet: Results of expression level analysis of mRNA, lncRNA, and miRNA. (A, B) The expression levels of different experimental groups were compared by violin and box diagrams of all transcripts of FPKM and TPM. For the same group of repeated samples, the final FPKM and TPM values were the average of all duplicated data. (C) Box diagrams were also created to show the expression levels of different types of transcripts. (D) Additionally, a comparative histogram was generated to show the number of exons in different types of transcripts.

Article Snippet: The expression of the selected mRNAs and miRNAs was quantitatively analyzed using a SYBR Green qPCR Mix Kit (TaKaRa) and miRcute Plus miRNA qPCR Kit (TIANGEN) according to the manufacturer’s instructions.

Techniques: Expressing, Generated

Journal: Frontiers in Endocrinology

Article Title: Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait

doi: 10.3389/fendo.2023.1165409

Figure Lengend Snippet: Differentially expressed genes (DEGs), lncRNA (DELs), and miRNAs (DEMs) in goat uterine. (A) Comparison of differentially expressed genes (DEGs) represented as a heatmap. (B) Comparison of differentially expressed lncRNA (DELs) represented as a heatmap. (C) Comparison of differentially expressed miRNAs (DEMs) represented as a heatmap. (D) Stacked column chart showing the distribution of DEGs, DELs, and DEMs in the comparison.

Article Snippet: The expression of the selected mRNAs and miRNAs was quantitatively analyzed using a SYBR Green qPCR Mix Kit (TaKaRa) and miRcute Plus miRNA qPCR Kit (TIANGEN) according to the manufacturer’s instructions.

Techniques: Comparison

Journal: Frontiers in Endocrinology

Article Title: Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait

doi: 10.3389/fendo.2023.1165409

Figure Lengend Snippet: Interaction regulation network about miRNA–lncRNA–mRNA. (A) Targeted mRNA is predicted based on differentially expressed miRNA. (B) Targeted lncRNA is predicted based on differentially expressed miRNA. (C) The ceRNA interaction network of lncRNA–miRNA–mRNA in which miRNA and lncRNA were screened according to the differentially expressed mRNA. The square represents miRNA, the blue outer ring circle represents lncRNA, and the yellow outer ring circle represents mRNA. The thickness of the wire represents the absolute value of the predicted interaction correlation, and the color inside the circle indicates the p-value.

Article Snippet: The expression of the selected mRNAs and miRNAs was quantitatively analyzed using a SYBR Green qPCR Mix Kit (TaKaRa) and miRcute Plus miRNA qPCR Kit (TIANGEN) according to the manufacturer’s instructions.

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait

doi: 10.3389/fendo.2023.1165409

Figure Lengend Snippet: Comparison of real-time quantitative PCR (RT–qPCR) and RNA-seq results for validation of differentially expressed DEGs (n=8) and DEMs (n=4). Relative gene expression was calculated using the 2 −△△CP method, FPKM (mRNA), and TPM (miRNA).

Article Snippet: The expression of the selected mRNAs and miRNAs was quantitatively analyzed using a SYBR Green qPCR Mix Kit (TaKaRa) and miRcute Plus miRNA qPCR Kit (TIANGEN) according to the manufacturer’s instructions.

Techniques: Comparison, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, RNA Sequencing, Biomarker Discovery, Gene Expression